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In Situ hybridization is the technique to use when you want to localize a specific mRNA or DNA species in tissue, cell or chromosomes. |
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Isotope labeled |
Digoxygenin labeled |
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| Isotope labeled probe such as S32 are commonly used when very high sensitivity is needed due to low copy number of target RNA in the observed tissue. | An good alternative to isotope labeled probe is digoxygenin labeled. The great advantage by not using radioactive material comes with a slight lower sensitivity. | |||||